Journal: Frontiers in Neuroscience

Article Title: Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

doi: 10.3389/fnins.2021.741279

Figure Lengend Snippet: Simultaneously recorded average VSD signal and LFP, both in barrel cortex, ECoG, and running speed in response to a facial air puff (one mouse). (A) A representative imaged cortical region: 375 × 375 μm 2 full-frame scan and correspondent 375 × 24 μm 2 box-scan imaged at 130 μm deep in the cortex. (B–D) VSD responses evoked by an air puff to the vibrissae and cheek of the mouse recorded in 375 × 24 × 5 μm 3 and 375 × 12 × 5 μm 3 box-scans from a single mouse at three different depths (B) during anesthesia (50 μm: 139 trials, 130 μm: 139 trials, and 350 μm: 138 trials), (C) in awake mouse during resting (50 μm: 127 trials; 130 μm: 125 trials; and 350 μm: 121 trials), and (D) in awake mouse during running (50 μm: 7 trials; 130 μm: 8 trials; and 350 μm: 12 trials). (E–G) LFP recorded in a single mouse by eight electrodes (every 4th channel of the linear probe, corresponding to a distance of 200 μm along the probe, Δdepth = 68 μm between adjacent traces). The probe was inserted with the tip at 850 μm below the dura. Each channel shown is the average of the same number of trials as for the imaging (light blue at 50 μm, blue at 130 μm, and dark blue at 350 μm). The bottom LFP corresponds to the electrode located 800 μm below the dura, the top LFP to the electrode located 100 μm below the dura. Corresponding (H–J) average ECoG and (K–M) average running speed. Dashed gray lines indicate the stimulus onset. All traces are plotted as mean ± standard deviation.

Article Snippet: Moreover, intracortical microelectrode arrays with metal electrodes, such as the commercially available Atlas Neuroengineering probes , enable the possibility to read small amplitude signals in the tens-microvolts to millivolts range, thus detecting fine electrophysiological traits such as single APs and multi-unit activity (MUA).

Techniques: Imaging, Standard Deviation

Journal: Frontiers in Neuroscience

Article Title: Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

doi: 10.3389/fnins.2021.741279

Figure Lengend Snippet: Average VSD signal 50 μm below dura and LFP, both in barrel cortex, ECoG, and running speed in response to a facial air puff (six mice). Average VSD responses evoked by an air puff to the vibrissae and cheek of the mouse in layer I (50 μm below dura) recorded in 375 × 24 × 5 μm 3 and 375 × 12 × 5 μm 3 box-scans in six mice (A) during anesthesia (133, 187, 139, 139, 126, 134 trials), (B) in awake mice during resting (111, 165, 118, 127, 104, 123 trials), and (C) in awake mice during running (16, 27, 14, 7, 16, 11 trials). (D–F) Corresponding average LFP recorded by eight electrodes (every 4th channel of the linear probe, tip at 850 μm below dura, bottom LFP trace: 800 μm, top LFP trace: 100 μm below dura, Δdepth = 68 μm between adjacent traces). Corresponding (G–I) average ECoG and (J–L) average speed. Each mouse is indicated by a different color; the overall average is shown in black. Gray dashed line marks the air puff onset.

Article Snippet: Moreover, intracortical microelectrode arrays with metal electrodes, such as the commercially available Atlas Neuroengineering probes , enable the possibility to read small amplitude signals in the tens-microvolts to millivolts range, thus detecting fine electrophysiological traits such as single APs and multi-unit activity (MUA).

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

doi: 10.3389/fnins.2021.741279

Figure Lengend Snippet: Average VSD signal 130 μm below dura and LFP, both in barrel cortex, ECoG, and running speed in response to a facial air puff (six mice). Average VSD responses evoked by an air puff to the vibrissae and cheek of the mouse in layer II (130 μm below dura) recorded in 375 × 24 × 5 μm 3 and 375 × 12 × 5 μm 3 box-scans in six mice (A) during anesthesia (135, 187, 139, 139, 130, 138 trials), (B) in awake mice during resting (109, 195, 125, 125, 96, 124 trials), and (C) in awake mice during running (19, 1, 7, 8, 13, 11 trials). (D–F) Corresponding average LFP recorded by 8 electrodes (every 4 th channel of the linear probe, tip at 850 μm below dura, bottom LFP trace: 800 μm, top LFP trace: 100 μm below dura, Δdepth = 68 μm between adjacent traces). Corresponding (G–I) average ECoG and (J–L) average speed. Each mouse is indicated by a different color; the overall average is shown in black. Gray dashed line marks the air puff onset.

Article Snippet: Moreover, intracortical microelectrode arrays with metal electrodes, such as the commercially available Atlas Neuroengineering probes , enable the possibility to read small amplitude signals in the tens-microvolts to millivolts range, thus detecting fine electrophysiological traits such as single APs and multi-unit activity (MUA).

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

doi: 10.3389/fnins.2021.741279

Figure Lengend Snippet: Average VSD signal 350 μm below dura and LFP, both in barrel cortex, ECoG, and running speed in response to a facial air puff (six mice). Average VSD responses evoked by an air puff to the vibrissae and cheek of the mouse in layer IV (350 μm below dura) recorded in 375 × 24 × 5 μm 3 and 375 × 12 × 5 μm 3 box-scans in 6 mice (A) during anesthesia (131, 189, 125, 138, 122, 136 trials), (B) in awake mice during resting (119, 174, 124, 121, 107, 112 trials), and (C) in awake mice during running (16, 5, 8, 12, 2, 22 trials). (D–F) Corresponding average LFP recorded by eight electrodes (every 4th channel of the linear probe, tip at 850 μm below dura, bottom LFP trace: 800 μm, top LFP trace: 100 μm below dura, Δdepth = 68 μm between adjacent traces). Corresponding (G–I) average ECoG and (J–L) average speed. Each mouse is indicated by a different color; the overall average is shown in black. Gray dashed line marks the air puff onset.

Article Snippet: Moreover, intracortical microelectrode arrays with metal electrodes, such as the commercially available Atlas Neuroengineering probes , enable the possibility to read small amplitude signals in the tens-microvolts to millivolts range, thus detecting fine electrophysiological traits such as single APs and multi-unit activity (MUA).

Techniques:

Journal: Frontiers in Neuroscience

Article Title: Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

doi: 10.3389/fnins.2021.741279

Figure Lengend Snippet: Average calcium signal at three different depth and LFP, both in barrel cortex, ECoG, and running speed in response to a facial air puff (four anesthetized and awake but resting mice). Average calcium signals recorded from three different depths in (A) anesthetized (4 mice, 50 μm: 236 trials, 55 ROIs, 130 μm: 221 trials, 44 ROIs, 400 μm: 228 trials, 50 ROIs) and awake but resting mice, the latter separated in (B) positive (4 mice, 50 μm: 213 trials, 42 ROIs; 130 μm: 227 trials, 42 ROIs, 400 μm: 215 trials, 38 ROIs) and (C) negative signals (4 mice, 50 μm: 213 trials, 15 ROIs; 130 μm: 227 trials, 20 ROIs, 400 μm: 215 trials, 20 ROIs). (D,E) Corresponding average LFP recorded by eight electrodes (every 4th channel of the linear probe, tip at 850 μm below dura, bottom LFP trace: 800 μm, top LFP trace: 100 μm below dura, Δdepth = 68 μm between adjacent traces). Corresponding (F,G) average ECoG and (H,I) average speed. All traces are plotted as mean ± standard deviation. Gray dashed line marks the air puff onset.

Article Snippet: Moreover, intracortical microelectrode arrays with metal electrodes, such as the commercially available Atlas Neuroengineering probes , enable the possibility to read small amplitude signals in the tens-microvolts to millivolts range, thus detecting fine electrophysiological traits such as single APs and multi-unit activity (MUA).

Techniques: Standard Deviation

Journal: Frontiers in Neuroscience

Article Title: Simultaneous Two-Photon Voltage or Calcium Imaging and Multi-Channel Local Field Potential Recordings in Barrel Cortex of Awake and Anesthetized Mice

doi: 10.3389/fnins.2021.741279

Figure Lengend Snippet: Average calcium signal at three different depth and LFP, both in barrel cortex, ECoG, and running speed in response to a facial air puff (four awake mice during running). Average calcium signals recorded from three different depths in awake mice during running, separated in (A) positive (4 mice, 50 μm: 24 trials, 32 ROIs; 130 μm: 9 trials, 37 ROIs; 400 μm: 25 trials, 30 ROIs) and (B) negative signals (4 mice, 50 μm: 24 trials, 24 ROIs, 130 μm: 9 trials, 12 ROIs, 400 μm: 25 trials, 28 ROIs). (C) Corresponding average LFP recorded by eight electrodes (every 4th channel of the linear probe, tip at 850 μm below dura, bottom LFP trace: 800 μm, top LFP trace: 100 μm below dura, Δdepth = 68 μm between adjacent traces. Corresponding (D) average ECoG and (E) average speed. All traces are plotted as mean ± standard deviation. Gray dashed line marks the air puff onset.

Article Snippet: Moreover, intracortical microelectrode arrays with metal electrodes, such as the commercially available Atlas Neuroengineering probes , enable the possibility to read small amplitude signals in the tens-microvolts to millivolts range, thus detecting fine electrophysiological traits such as single APs and multi-unit activity (MUA).

Techniques: Standard Deviation